Getting started with Muscle: Multiple Protein Sequence Alignment  Tool 

Author Name : C.P.Rajadurai

Mail ID : rajaduraicp_at_au-kbc.org


Muscle is a tool for doing multiple sequence alignment between protein sequences. 

Step: 1 

Change the directory to folder containing sequences and type muscle and enter. 

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Step: 2

Give the input by typing muscle –in sequencefilename –out outputfilename and press enter.

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Step: 3 
 

The result page shows the percentage of identity and the aligned sequences.

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Step: 4 

View the output file by typing cat outputfilename.txt and press enter. 

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